Capillary electrophoresis of proteins 2003–2005
نویسندگان
چکیده
منابع مشابه
Capillary Electrophoresis of PEGylated Proteins
Capillary electrophoresis has become a well-established technique for evaluating the composition and concentrations of a wide range of protein samples. In addition, capillary electrophoresis can be used to study a variety of protein properties including the net protein charge, the extent of ion binding, and the presence of specific biomolecular interactions. This wide range of applications is p...
متن کاملCapillary electrophoresis of proteins and peptides.
Capillary electrophoresis (CE) is a high-resolution technique for the separation of a wide variety of molecules of biological interest such as metabolites, drugs, amino acids, nucleic acids, and carbohydrates. This unit focuses on the use of CE to separate proteins and peptides based on charge-to-mass ratios. Separation of proteins based on their isoelectric points is described along with a pro...
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Cereal grains are widely used of human foods and animal feed throughout the world. Cereals provide dietary protein, which also often has a functional role, as wheat gluten does in bread. Cereal proteins are unique in many ways: they are highly complex and heterogeneous, are often difficult to extract, and aggregate readily, making them difficult to characterize. Because of the economic importan...
متن کاملNoncovalent polycationic coatings for capillaries in capillary electrophoresis of proteins.
The adsorption of proteins with net positive charges (pI > pH) on the walls of fused-silica capillaries is a common problem in the analysis of proteins by capillary electrophoresis. This paper explores the use of polycationic polymers as noncovalent coatings to limit this problem. The behavior of three sets of proteins was compared using uncoated and coated capillaries: (i) a protein charge lad...
متن کاملQuantitative analysis of serum proteins separated by capillary electrophoresis.
The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samp...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2006
ISSN: 0173-0835,1522-2683
DOI: 10.1002/elps.200500567